NFkB, p65 (Transcription Factor p65, Nuclear Factor NF-kappa-B p65 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B Cells 3, NFKB3, RELA)
Produktgrößen
100ug
£819,00
N2303-10K-100UG
Über dieses Produkt
- SKU:
- N2303-10K
- Anwendung:
- Flow Cytometry
- translate.label.attr.clone:
- 12K142
- Klonalität:
- Monoclonal
- Weitere Details:
- RelA p65 is a 65kD member of the NFkB family of nuclear transcription factors. Dimers of p65 with the p50 subunit are the most common form of the NFkB transcription factor, but dimers with itself or other family members are also active. An alternatively spliced isoform that lacks aa222-231 (p65D) does not bind DNA. Over the sequence used as an immunogen, human RelA p65 shares 96% and 98% aa identity with mouse and rat RelA p65, respectively. This portion includes one of eight potential Ser/Thr phosphorylation sites, two acetylation sites, and most of the Rel homology domain that interacts with IkB inhibitors. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug labels/10e6 in Daudi human Burkitt's lymphoma cell line fixed with paraformaldehyde and permeabilized with methanol. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- Recombinant corresponding to aa1-254 from human RelA expressed in E.coli.
- Isotyp:
- IgG2a
- Physischer Zustand:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human NFkB p65.
- Quelle:
- human
- Lagerbedingungen:
- 4[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody