Neuron Specific Enolase, gamma (gNSE) (HRP)
Produktgrößen
100ul
£1099,00
N2175-09-HRP-100UL
Über dieses Produkt
- SKU:
- N2175-09-HRP
- Anwendung:
- Immunohistochemistry
- translate.label.attr.clone:
- 1.B.652
- Klonalität:
- Monoclonal
- Weitere Details:
- Enolases are homo- or heterodimers of the three subunits: alpha, 46kD, beta, 44kD, and gamma, 46kD. The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Formalin-fixed and paraffin-embedded sections. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Purified neuron-specific enolase from human brain.
- Isotyp:
- IgG2b
- Physischer Zustand:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).
- Reinheit:
- Purified by Protein G affinity chromatography. Purity is tested by electrophoresis.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Specific for human gamma enolase subunit. No detectable crossreactivity with the alpha or beta subunits. Reacts with 45kD band corresponding to NSE in Immunoblot of human brain extracts.
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody