MEF2C (Myocyte-specific Enhancer Factor 2C)
Produktgrößen
100ug
£876,00
M9769-97L-100UG
Über dieses Produkt
- SKU:
- M9769-97L
- Anwendung:
- Western Blot
- Klonalität:
- Polyclonal
- Weitere Details:
- MEF2C is a transcriptional activator that is a member of the MEF2 subfamily, MADS (MCM1, Agamous, Deficiens, Serum response factor) gene family of proteins. Although its predicted MW is 51kD, it runs anomalously at 57kD in SDS-PAGE. It is expressed in B cells, plus postmitotic neurons and skeletal muscle cells that are undergoing differentiation. Human MEF2C is 473aa in length. It contains a MADS box for dimerization aa3-57, a DNA binding domain aa58-86, a beta-domain that enhances transcription aa271-278, a gamma-domain that, when phosphorylated, promotes transcriptional repression aa390-399, and seven acetylation sites plus multiple Ser/Thr phosphorylation sites. Sumoylation occurs on Lys391, and proteolytic cleavage of MEF2C occurs in neurons between aa432-433. Multiple splice forms exist. Either individually or in combination, there may be a deletion of aa271-278, 368-399 or 87-134, a 46aa substitution for aa107-134 and 87-134, and an alternative start site 35aa upstream of the standard site. Over aa135-239, human MEF2C shares 96% aa identity with mouse MEF2C. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to aa135-239 from human MEF2C expressed in E. coli.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Reinheit:
- Purified by immunoaffinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human MEF2C.
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody