LRP1B (Low-density Lipoprotein Receptor-related Protein 1B, LRP-1B, LRPDIT)
Produktgrößen
100ug
£876,00
L2601-05S-100UG
Über dieses Produkt
- SKU:
- L2601-05S
- Anwendung:
- Western Blot
- Klonalität:
- Polyclonal
- Weitere Details:
- LRP-1B is a 600-620kD member of the LDLR family of proteins. It is expressed on vascular smooth muscle cells and likely neurons, and binds multiple molecules,including uPA,PAI-1,PDGFRb, uPAR, and Pseudomonasexotoxin.Itisconsideredatumor suppressor, and appears to block the generation of plasmin. Mature human LRP-1B is a 4579aa type I transmembrane (TM) glycoprotein. It contains a 4424aa extracellular domain (ECD) aa21-4444 plus a 132aa cytoplasmic region aa4468-4599. In the ECD, there are 32 LDLR class A repeats, 14 EGF-like repeats, and 36 LDLR class B repeats that show an interspersing pattern. In the cytoplasmic region, one NLS aa4468-4474 and two endocytosis signal motifs aa4492-4562 are found. LRP-B1 is cleaved in the Golgi by furin after Arg3957 to create a noncovalent heterodimer consisting of a 515kD ECD, and an 85-95kD TM segment. Either uncleaved LRP-1B, or the dissociated heterodimer can undergo additional processing, leading to cleavage of the 90kD TM segment after Lys4435. This creates a 21kD TM C-terminal fragment that can undergo further g-secretase processing to generate a cytoplasmic, 18kD peptide that translocates to the nucleus. Over aa21-244, human LRP-1B shares 81% aa identity with mouse LRP-1B. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml on human liver tissue lysates Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Leu21-Glu244 from human LRP-1B expressed in E. coli.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Reinheit:
- Purified by immunoaffinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human LRP-1B. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) LRP‑1, rhLRP‑4, rhLRP‑5, and rhLRP‑6 is observed.
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody