Latency Associated Peptide, Transforming Growth Factor beta1 (LAP, TGFb1) (PE)
Produktgrößen
100Tests
£806,00
L1393-30-100TESTS
Über dieses Produkt
- SKU:
- L1393-30
- Anwendung:
- Flow Cytometry
- translate.label.attr.clone:
- 7H66
- Klonalität:
- Monoclonal
- Weitere Details:
- TGF-beta is secreted by cells in the form of an inactive complex. This complex consists of TGF-beta associated non-covalently with a protein designated the latency associated peptide (LAP). TGF-beta and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-beta each consist of a disulfide-linked homodimer and the association of these two components renders TGF-beta inactive and inaccessible to anti-TGF-beta antibodies. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels ≤1x10e6 cells. Validated using CD4+CD25+ human PBMCs and mouse splenocytes. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human or mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking add 10ul of L1393-30 to ≤1x10e6 cells and incubate for 30 minutes at RT. 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (Catalog # F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human or Mouse Lyse Buffer. 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Leu30-Arg278 (Cys33Ser) of human LAP (TGF-b1) expressed in S. frugiperda insect ovarian cell line Sf 21 (P01137).
- Isotyp:
- IgG1
- Physischer Zustand:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Reinheit:
- Purified by Protein G affinity chromatography from culture supernatant.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human LAP (TGFb1). Species Crossreactivity: Mouse
- Quelle:
- human
- Lagerbedingungen:
- 4[o]C Do not freeze
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody