Interleukin 11 (IL-11) (Biotin)
Produktgrößen
25ug
£479,00
I8433-03-25UG
Über dieses Produkt
- SKU:
- I8433-03
- Anwendung:
- Western Blot
- Klonalität:
- Polyclonal
- Weitere Details:
- TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Sandwich ELISA: 0.25-1.0ug/ml, using 100ul/well in conjunction with I8433-10A as a capture antibody, allows the detection of at least 2000-4000pg/ml of recombinant human IL-11 Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human IL-11, expressed in E. coli.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a lyophilized powder from PBS, pH 7.4. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA to a concentration of 0.1-1mg/ml.
- Reinheit:
- Purified by immunoaffinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human Interleukin-11 (IL-11).
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody