IL6ST (Interleukin-6 Receptor Subunit beta, IL-6 Receptor Subunit beta, IL-6R Subunit beta, IL-6R-beta, IL-6RB, Interleukin-6 Signal Transducer, Membrane Glycoprotein 130, gp130, Oncostatin-M Receptor Subunit alpha, CD130)
Produktgrößen
100ug
£876,00
I8428-39R-100UG
Über dieses Produkt
- SKU:
- I8428-39R
- Anwendung:
- Western Blot
- Klonalität:
- Polyclonal
- Weitere Details:
- Glycoprotein 130 is a ubiquitously expressed, 130kD type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane. Rat gp130 is synthesized as a 918aa precursor with a 22aa signal sequence, a 596aa extracellular domain (ECD), a 22aa transmembrane region, and a 278aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD. The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains. The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family. Rat gp130 shares 88% and 79% aa sequence identity with mouse and human gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1, IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to aa23-618 from rat GP130 expressed in NSO cell line.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Reinheit:
- Purified by immunoaffinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes rat GP130. Species Crossreactivity: Recombinant mouse gp130 (~45%), recombinant human gp130 (~6%).
- Quelle:
- rat
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody