HLA Class 1 Antigen A33,B8
Produktgrößen
100ul
£1727,00
H6098-39-100UL
Über dieses Produkt
- SKU:
- H6098-39
- translate.label.attr.clone:
- 4i104
- Klonalität:
- Monoclonal
- Weitere Details:
- The human leukocyte antigen system (HLA) is the name of the major histocompatibility complex (MHC) in humans and is an essential element for immune function. This group of genes re- sides on chromosome 6, and encodes cell-surface antigen- presenting proteins and many other genes. Different classes have different functions: HLA class I antigens (A, B & C) present peptides from inside the cell, including viral peptides if present. These peptides are produced from digested proteins that are broken down in the proteasomes. Foreign antigens attract killer T-cells (also called CD8+ or cytotoxic T-cells) that destroy cells. Applications: This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies. Recommended Dilutions: Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution. Optimal dilutions to be determined by the researcher. Cytotoxicity: Expected Results: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Isotyp:
- IgM
- Physischer Zustand:
- Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.
- Reinheit:
- Ascites.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes HLA Class 1 Antigen A33,B8. Specificity was determined at a 1:10 dilution by the Microcytotoxicity Assay under standard NIH conditions.
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody