Ghrelin (Not for Export EU)
Produktgrößen
100ul
£704,00
G2033-75D-100UL
Über dieses Produkt
- SKU:
- G2033-75D
- Klonalität:
- Polyclonal
- Weitere Details:
- Growth hormone (GH), produced and released from the anterior pituitary, controls body growth, carbohydrate-protein-lipid metabolism and water-electrolyte balance. GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release. GHSs, act through a novel orphan G-protein-coupled receptor (GPCR), the GHS receptor (GHS-R). Ghrelin ('ghre' is the Proto-Indo-European root of the word "grow") has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R. The rat and human mature Ghrelin (28-aa) are produced from 117 amino acids precursor. In rat stomach, a 28-aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA. The activity of both Ghrelins is the same. However, des-Gln14-Ghrelin is only present in low amounts in the stomach, indicating that Ghrelin is the major active form. Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity. Ghrelin is the first known example of a bioactive peptide modified by an acyl acid. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 (ECL) ELISA: 1:10,000-1:100,000. Using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: G2033-75F: Ghrelin, Human Control Peptide Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to a 13aa sequence that is n-octanoylated at Ser3, from the N-terminus of mature human ghrelin (KLH). Species sequence homology: porcine; 94%; bovine, canine, mouse and rat; 84%.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Reinheit:
- Serum
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human ghrelin.
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody