FGF-10, Recombinant, Human (Fibroblast Growth Factor-10, FGFA, Keratinocyte growth factor-2)
Produktgrößen
25ug
£492,00
F4212-05A-25UG
Über dieses Produkt
- SKU:
- F4212-05A
- Weitere Details:
- FGF-10 is a heparin-binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-10 is most related to KGF/FGF-7, and is expressed during the development and, preferentially, in adult lungs. It signals through FGFR 2b. Recombinant Human FGF-10 is a 19.3kD protein consisting of 170 amino acid residues. Recombinant corresponding to human Fibroblast Growth Factor 10 expressed in E. coli. Molecular Weight: ~19.3kD Amino Acid Sequence: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS Biological Activity: Assay #1: Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using cell counting Kit to CCK-8. The expected ED50 is 5ng/ml corresponding to a specific activity of ≥ 2x10e6 units/mg. Assay #2: Determined by a cell proliferation assay using 4MBr-5 cells. The expected ED50 for this effect is 0.5-15ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile 5mM sodium phosphate, pH 7.4. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molekulargewicht:
- 19.3
- Physischer Zustand:
- Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute in 5mM sodium phosphate, pH 7.4 to 0.1-1mg/ml. Further dilutions should be made in aqueous buffers supplemented with carrier protein, su
- Reinheit:
- ≥95% (SDS-PAGE, HPLC). Endotoxin: ≤0.1ng/ug (≤1EU/ug)
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins