Cytokeratin 20
Produktgrößen
50ug
£800,00
C9097-29D-50UG
Über dieses Produkt
- SKU:
- C9097-29D
- Anwendung:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- IT-Ks20.8
- Klonalität:
- Monoclonal
- Weitere Details:
- Represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Applications: Suitable for use in Immunohistochemistry (paraffin sections after microwave treatment) and Immunoblot (Western). Recommended Dilution: Immunohistochemistry: 1:10 in PBS (pH 7.4) (1:100 when applying the microwave method); Incubation Time 1 hr at RT or overnight at 2-8°C. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Electrophoretically purified cytokeratin (CK) 20 from human intestinal mucosa.
- Isotyp:
- IgG2a
- Physischer Zustand:
- Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA and 0.09% sodium azide. Reconstitute to a final volume of 1ml.
- Reinheit:
- Purified by Protein A affinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human Cytokeratin 20. Does not react with rat and mouse. Reactivities on Cultured HT-29, LoVo, DLD-1, SW 1116. Polypeptide recognized: protein IT (cytokeratin No. 20; Mr 46,000). Cell Lines (tested so far) CaCo-2, RT-4.
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody