Corticotropin Releasing Hormone Binding Protein (CRHBP)
Produktgrößen
100ug
£767,00
C7903-99A-100UG
Über dieses Produkt
- SKU:
- C7903-99A
- Anwendung:
- Western Blot
- translate.label.attr.clone:
- 7H201
- Klonalität:
- Monoclonal
- Weitere Details:
- Human CRHBP is a 37kD secreted glycoprotein that likely serves to regulate CRH availability to the adrenal. In human, CRHBP complexes with CRH to form a circulating 41kD complex. In rodent and horse, this complex is restricted to the CNS. Human CRHBP is synthesized as a 322 amino acid (aa) precursor that contains a 24 aa signal sequence and a 298 aa mature region. The mature region contains one potential CUB domain and five intrachain disulfide bonds. Human mature CRHBP shares 87% and 84% sequence identity with mouse and equine CRHBP, respectively. Applications: Western Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human CRHBP. Using a colorimetric detection system, the detection limit for rhCRHBP is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human CRHBP. The detection limit for rhCRHBP is approximately 15 ng/well. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- NS0-derived recombinant protein corresponding to Tyr25-Leu322 from human CRHBP.
- Isotyp:
- IgG1
- Physischer Zustand:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Reinheit:
- Purified by Protein G affinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Selected for its ability to detect human CRHBP in direct ELISAs and Western Blots. In these applications, this antibody shows no crossreactivity with rmCRHBP.
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody