CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (PE/CY5)
Produktgrößen
100ug
£381,00
C2400-05F-100UG
Über dieses Produkt
- SKU:
- C2400-05F
- Anwendung:
- Immunoprecipitation, Flow Cytometry, Immunohistochemistry
- translate.label.attr.clone:
- 13/2.3
- Klonalität:
- Monoclonal
- Weitere Details:
- CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes.1-4 CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells.4 Applications: • Flow cytometry 2, 3 • Immunoprecipitation 1, 3 • Radiometric quantitation of CD45 molecules on cell surfaces 1 • Immunohistochemistry (frozen sections) Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
- Host:
- Rat
- Isotyp:
- IgG2b
- Physischer Zustand:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
- Reinheit:
- Purified
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes mouse CD45 (T200 or leukocyte common antigen/LCA, all isoforms). Recognizes a framework epitope present on all CD45 isoforms.
- Quelle:
- mouse
- Lagerbedingungen:
- 4[o]C Do Not Freeze
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody