Caspase 4 (Caspase-4, CASP 4, CASP4, CASP-4, Apoptotic Cysteine Protease Mih1/TX, ICH 2, Ich-2, ICH2 Protease, Mih1/TX, TX, TX Protease) (Biotin)
Produktgrößen
100ul
£947,00
C2087-23V-BIOTIN-100UL
Über dieses Produkt
- SKU:
- C2087-23V-BIOTIN
- Anwendung:
- Western Blot, Immunohistochemistry
- Klonalität:
- Polyclonal
- Weitere Details:
- Caspase-4, a member of the Caspase-1 subfamily of cysteine proteases, exists as an inactive pro-enzyme which undergoes proteolytic cleavage of its p30 precursor form, into smaller p20 and p10 subunits. The cellular localisation of Caspase-4 on the Endoplasmic Reticulum (ER) membrane, has resulted in this protein becoming a focus of studies in which dysfunction or stress to the ER membrane is implicated, such as Alzheimer’s disease and Ischemia (1, 3) and confirms the involvement of Caspase-4 as an instigator of cellular apoptosis. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): Does not require protein digestion pre-treatment or antigen retrieval using heat treatment prior to staining paraffin sections. Western Blot: Detects a band at ~34kD in Ramos cell lysates. Optimal dilutions to be determined by the researcher. Positive Control: Spleen tissue Recommended Secondary Antibody: I1903-30U: IgG, H&L, X-adsorbed (HRP) Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to a 16aa from human Caspase-4 at N-terminal. Epitope: aa20-70
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Reinheit:
- Purified by immunoaffinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes the N-terminal of human Caspase-4. Species Crossreactivity: mouse
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody