Caspase 4 (Caspase-4, CASP 4, CASP4, CASP-4, Apoptotic Cysteine Protease Mih1/TX, ICH 2, Ich-2, ICH2 Protease, Mih1/TX, TX, TX Protease) (APC)
Produktgrößen
100ul
£947,00
C2087-23V-APC-100UL
Über dieses Produkt
- SKU:
- C2087-23V-APC
- Anwendung:
- Western Blot, Immunohistochemistry
- Klonalität:
- Polyclonal
- Weitere Details:
- Caspase-4, a member of the Caspase-1 subfamily of cysteine proteases, exists as an inactive pro-enzyme which undergoes proteolytic cleavage of its p30 precursor form, into smaller p20 and p10 subunits. The cellular localisation of Caspase-4 on the Endoplasmic Reticulum (ER) membrane, has resulted in this protein becoming a focus of studies in which dysfunction or stress to the ER membrane is implicated, such as Alzheimer’s disease and Ischemia (1, 3) and confirms the involvement of Caspase-4 as an instigator of cellular apoptosis. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): Does not require protein digestion pre-treatment or antigen retrieval using heat treatment prior to staining paraffin sections. Western Blot: Detects a band at ~34kD in Ramos cell lysates. Optimal dilutions to be determined by the researcher. Positive Control: Spleen tissue Recommended Secondary Antibody: I1903-30U: IgG, H&L, X-adsorbed (HRP) Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to a 16aa from human Caspase-4 at N-terminal. Epitope: aa20-70
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Reinheit:
- Purified by immunoaffinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes the N-terminal of human Caspase-4. Species Crossreactivity: mouse
- Quelle:
- human
- Lagerbedingungen:
- 4[o]C Do Not Freeze
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody