Catenin, gamma (Plakoglobin) (APC)
Produktgrößen
100ul
£1106,00
C2069-60-APC-100UL
Über dieses Produkt
- SKU:
- C2069-60-APC
- Anwendung:
- Immunoprecipitation, Western Blot, Immunofluorescence
- translate.label.attr.clone:
- 3G62
- Klonalität:
- Monoclonal
- Weitere Details:
- Plakoglobin (gamma-catenin) is an 83kD protein that localizes to the cytoplasmic face of both desmosomal and adherens junctions. Plakoglobin was first demonstrated to associate with the desmosomal cadherins, desmoglein and desmocollin, and was subsequently shown to associate with the classical cadherins E- and N-cadherin. Plakoglobin’s interaction with E-and N-cadherin lead to its identification as the ~80kD protein originally called gamma-catenin. Recent evidence indicates that the classical and desmosomal cadherins actually interact with distinct domains of the plakoglobin protein. Further, the desmosomal cadherins have been shown to regulate the accumulation of plakoglobin in the cell by decreasing its turnover rate. Plakoglobin (g-catenin) is highly homologous to both b-catenin and the product of the Drosophila segment polarity gene armadillo. Plakoglobin (g-catenin) and b-catenin contain 13 copies of a 42–44 amino acid motif first identified in the Armadillo protein and referred to as armadillo repeats. These armadillo repeats not only mediate the interaction between b and g-catenin and the cadherin cytoplasmic domain, but are also responsible for interactions with other cellular proteins. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells, A431 cells, WI-38 human fibroblasts Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Fusion protein consisting of the maltose binding protein fused to full length human plakoglobin protein. Epitope has been localized to a site between aa45-114 of the human plakoglobin protein.
- Isotyp:
- IgG1,k
- Physischer Zustand:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Reinheit:
- Purified by Protein A affinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human plakoglobin at ~83kD. Does not appear to crossreact with the related b-catenin protein (~92kD). Species Crossreactivity: mouse
- Quelle:
- human
- Lagerbedingungen:
- 4[o]C Do Not Freeze
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody