BMP8B (Bone Morphogenetic Protein 8B, BMP-8B)
Produktgrößen
100ug
£876,00
B2553-35X-100UG
Über dieses Produkt
- SKU:
- B2553-35X
- Anwendung:
- Western Blot
- Klonalität:
- Polyclonal
- Weitere Details:
- BMP-8b is a 16kD predicted mature and 45kD precursor member of the Gbb-60A class of the BMP family of molecules. In mouse, precursors to sperm and oocytes arise from the proximal epiblast around day E7.5 (days 18-20 in human). This lineage commitment is a response to BMP-4 signaling, which is promoted by the repressive action of BMP-8b on the anterior visceral endoderm, a source of BMP-4 antagonism. Mouse BMP-8b preproprecursor is 399aa in length. It contains a 19aa signal sequence, a 241aa prosegment, and a 139aa mature region. An interchain disulfide bond occurs at 363aa, generating a homodimer. Over aa261-399, mouse BMP-8b shares 74% aa identity with mouse BMP-8a, and 69% and 93% aa identity with human and rat BMP-8b. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to aa261-399 from mouse BMP-8b expressed in E.coli.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Reinheit:
- Purified by immunoaffinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes mouse BMP-8b. Species sequence homology: Recombinant human BMP-3b, rhBMP-10, and recombinant mouse BMP-10 human (10%).
- Quelle:
- mouse
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody