Betaine-Homocysteine Methyltransferase (BHMT, Betaine-Homocysteine S-Methyltransferase)
Produktgrößen
100ul
£576,00
B1082-12B-100UL
Über dieses Produkt
- SKU:
- B1082-12B
- Anwendung:
- Western Blot
- translate.label.attr.clone:
- 7G21
- Klonalität:
- Monoclonal
- Weitere Details:
- Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulfur amino acids, choline and betaine. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Recombinant human BHMT (1-406aa) (E. coli)
- Isotyp:
- IgG2a,k
- Physischer Zustand:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Reinheit:
- Purified by Protein G affinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human BHMT at ~45kD. Species Crossreactivity: Mouse.
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody