AP-2
Produktgrößen
100ug
£709,00
A2298-59-100UG
Über dieses Produkt
- SKU:
- A2298-59
- Anwendung:
- Western Blot, Immunofluorescence, Radioimmunoassay, Immunohistochemistry, Flow Cytometry
- translate.label.attr.clone:
- A6/2/2
- Klonalität:
- Monoclonal
- Weitere Details:
- AP-2 is a sequence-specific DNA binding transcription factor first identified in mammalian cultured cells. In humans and mice, AP-2 belongs to a gene family that includes two other genes, AP-2b and AP-2.2. The AP-2 family proteins share a novel, highly conserved DNA binding and dimerization domain referred to as a basic helix-span-helix domain in their C-terminal halves, while their N-terminal portions have diverged considerably. AP-2 transcription factors have been suggested to exert key regulatory functions in vertebrate embryonic development, in tumorigenicity of various cancer cell types. The AP-2 gene family also plays a role in the control of cell growth and differentiation in breast cancer. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, RIA, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:2000 ELISA: 1:100-1:2000 Flow Cytometry: 1:10-1:1000 Immunocytochemistry (IF-IC): 1:10-1:500 Immunohistochemistry: 1:10-1:500, using formal saline fixative with calcium on paraffin embedded specimens (10min microwave treatment required). RIA: 1:100-1:2000 Optimal dilution determined by the researcher. Positive Control: Raji cells or breast carcinomas. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Peptide corresponding to PNSHTDNNAKSSDKEEKHRK from the C-terminal of AP-2 Alpha (KLH).
- Isotyp:
- IgG1
- Physischer Zustand:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Reinheit:
- Purified by Protein A affinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes both the alpha and beta forms of AP2. Species Crossreactivity: human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody