Actin, Cardiac, Fetal
Produktgrößen
50ug
£800,00
A0759-25-50UG
Über dieses Produkt
- SKU:
- A0759-25
- Anwendung:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 5F152
- Klonalität:
- Monoclonal
- Weitere Details:
- Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbilical cord. Applications: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:500 (see Note below). Western Blot: 1:1000 (using ECL) Note: It is necessary to include 1M sodium chloride for application in Western Blot and ELISA to enhance specificity. Immunohistochemistry (Frozen, Paraffin): 1:10. It is necessary to include 0.5M sodium chloride in all washing buffers for application on frozen tissue. This is not necessary for paraffin sections. Microwave treatment is necessary for paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O/PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Synthetic N-terminus decapeptide of cardiac isoform of actin.
- Isotyp:
- IgG1
- Physischer Zustand:
- Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
- Reinheit:
- Purified by Protein G affinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes fetal cardiac actin. Represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) a-actin from all other actin isoforms. Shows no crossreaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied. Species Crossreactivity: human, bovine, rabbit and chicken
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody