Acid Sensing Ion Channels 4 (ASIC4) (Not for Export EU)
Produktgrößen
100ul
£704,00
A0577-100UL
Über dieses Produkt
- SKU:
- A0577
- Klonalität:
- Polyclonal
- Weitere Details:
- Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+ -gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC); (3) mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride. The mammalian homolog of degenerins (MDEG or MDEG1; now designated ASICs for Acid Sensing Ion Channels). Three are at least 4 distinct proteins in ASIC family ASIC1-4. AISC4/SPASIC (human 539/666aa, chromosome 2; rat 539aa, ~97% homology between rat and human) is strongly expressed in pituitary and also detected in most areas of brain. ASIC4 is the most divergent member of ASIC family showing only 45% identity with ASIC1-3. ASIC4 is not activated by low pH. It may associate with other ASICs to form a functional channel. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 ELISA: 1:10,000-50,000 Optimal dilutions to be determined by the researcher. Control Peptide: A0577-10 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide 19aa sequence of human ASIC4 (KLH coupled). Location: Extracellular.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid, serum, 0.05% sodium azide, 40% glycerol.
- Reinheit:
- Serum
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes Acid Sensing Ion Channels 4. No significant sequence homology is detected with other ASIC-beta or other ASICs. Species Sequence Homology: Rat: 100%.
- Quelle:
- human
- Lagerbedingungen:
- 4[o]C (-20[o]C Glycerol)
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody