NUP50 (Nucleoporin 50kD, NPAP60, NPAP60L, Nuclear pore-associated protein 60kD-like)
Produktgrößen
200ul
£473,00
364458-200UL
Über dieses Produkt
- SKU:
- 364458
- Anwendung:
- Western Blot, Immunohistochemistry
- Klonalität:
- Polyclonal
- Weitere Details:
- Nucleoporin 50kD is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. NUP50 is a tristable switch that alternates between binding modes. The C terminus binds importin-beta through RAN-GTP. The N terminus binds the C terminus of importin-alpha, while a central domain binds importin-beta. NUP50:importin-alpha:importin-beta binds cargo and can stimulate nuclear import. Endogenous NUP50 can shuttle and is accessible from the cytoplasmic side of the nuclear envelope. These findings identified NUP50 as a cofactor for importin-alpha:importin-beta nuclear import and as a subunit of the importin complex. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.01-2ug/ml Immunohistochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Pro179-Val428 foil or amber vial rat NUP50, expressed in E. coli.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Reinheit:
- Purified by immunoaffinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes rat NUP50.
- Quelle:
- rat
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody