NUAK1 (NUAK Family SNF1 Like Kinase 1, ARK5, OMPHK1, NuaK1, AMP-Activated Protein Kinase Family Member 5, Omphalocele kinase 1)
Produktgrößen
200ul
£544,00
364442-200UL
Über dieses Produkt
- SKU:
- 364442
- Anwendung:
- Western Blot, Immunohistochemistry
- Klonalität:
- Polyclonal
- Weitere Details:
- The deduced 661aa protein shares about 52% identity over 252aa with plant SNF1-related protein kinase. NUAK1 had an apparent molecular mass of about 75kD by SDS-PAGE. RT-PCR detected highest expression in heart and brain, followed by skeletal muscle, kidney, ovary, placenta, lung, and liver. Little to no expression was detected in other tissues examined. The phosphorylation was stimulated by AMP and did not require accessory binding proteins. Transfection of ARK5 also increased the survival of HepG2 cells exposed to glucose starvation and reduced oxygen tension. Increased cell survival was accompanied by phosphorylation of ARK5 on ser600 by AKT, which activated ARK5 kinase activity. Activated ARK5 phosphorylated ATM, which led to phosphorylation of p53. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.01-2ug/ml. mmunohistochemistry: 5-20ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa404-651 of mouse NUAK1, expressed in E. coli.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes mouse NUAK1. Species Crossreactivity: rat
- Quelle:
- mouse
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody