Syk (Tyrosine-protein Kinase SYK, Spleen Tyrosine Kinase) (Pacific Blue®)
Produktgrößen
100ul
£748,00
351927-100UL
Über dieses Produkt
- SKU:
- 351927
- Anwendung:
- Flow Cytometry
- translate.label.attr.clone:
- D3Z1E
- Klonalität:
- Monoclonal
- Weitere Details:
- Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCgamma1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (9). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Caution: Pacific Blue® conjugates are sensitive to light.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues around Asn463 of human Syk.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with Pacific Blue®.
- Reinheit:
- Purified
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human Syk. Species Crossreactivity: mouse and rat
- Quelle:
- human
- Lagerbedingungen:
- 4[o]C Do Not Freeze
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody