SCF, Recombinant, Human, Animal Free (Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor, MGF, Steel Factor)
Produktgrößen
10ug
£448,00
208690-10UG
Über dieses Produkt
- SKU:
- 208690
- Weitere Details:
- SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Human SCF is an 18.4kD polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF. Recombinant protein corresponding to human Stem Cell Factor, expressed in E. coli. Amino Acid Sequence: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2ng/ml, corresponding to a specific activity of ≥ 5x10e5 units/mg. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile acetic acid. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molekulargewicht:
- 18.4
- Physischer Zustand:
- Supplied as a lyophilized powder from 10mM acetic acid. Reconstitute with sterile ddH2O to a concentration ≥0.1mg/ml. For long term storage add 0.1% HSA or BSA.
- Reinheit:
- ≥98% (HPLC, SDS-PAGE). Endotoxin: ≤0.01ng/ug (0.1EU/ug).
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins