Sm (Smith) (Not For Export EU)
Produktgrößen
1000U
£1321,00
208590-1000U
Über dieses Produkt
- SKU:
- 208590
- Weitere Details:
- Sm antigen is a non-histone nuclear protein composed of several polypeptides of differing molecular weights. They include B (26 kD), B'(27 k D), and D (13 kD). The principle reactivity has been shown to reside in the B, B', and D polypeptides (3). Anti-Sm autoantibodies were described originally as precipitating autoantibodies in sera of patients with Systemic Lupus Erythematosus (1). Anti-Sm antibodies are also usually accompanied by antinuclear ribonucleoprotein (nRNP) antibodies (2). The U1 RNP particle has both Sm and RNP binding specificities. The difference is that the RNP particles bound by U2, U4/6 and U5 are bound by anti-Sm autoantibodies, but not by anti-nRNP autoantibodies. Autoantibodies against the Sm antigen precipitate the U1, U2, U4/6, and U5, small nuclear RNAs (4). The Sm antigen is involved in normal post-transcriptional, premessenger RNA processing to excise introns (5). It has been demonstrated that the Sm antigenicity is both RNase and DNase resistant and partially resistant to tryptic digestion (6). Autoantibodies to Sm antigen have been observed in 15 to 30% of SLE sera as a diagnostic marker. (7) It is thought that IgG anti-Sm correlates with Lupus disease activity and is a useful variable in predicting exacerbation and prognosis of SLE. (8) It has been reported that IgG anti-Sm is specifically detected in patients with SLE. IgM anti-Sm has rarely been detected in SLE and was recognized in other diseases (2). It has also been reported in a study of clinical significance, that the frequency of lung fibrosis and pericarditis was significantly higher in patients with IgG, IgA, and/or IgM anti-Sm (2). Unit Definition: The amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedure for enzyme immunoassay. Activity will vary with assay conditions. Applications: Recommended for use in standard solid phase immunoassays. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Physischer Zustand:
- Supplied as a liquid in 0.01M Tris, 0.53M sodium chloride, 0.02% sodium azide.
- Reinheit:
- Purified
- Versandbedingungen:
- Dry Ice
- Lagerbedingungen:
- -70[o]C
- Typ:
- Buffers & General Consumables:Others