Cluster Of Differentiation 40 Ligand (CD40L) Recombinant, Porcine
Produktgrößen
10ug
£367,00
154029-10UG
Über dieses Produkt
- SKU:
- 154029
- Weitere Details:
- Recombinant protein corresponding to aa111-261 with an N-Terminal His Tag from porcine CD40L, expressed in E. coli. Uniprot/Accession: Q95MQ5 Amino Acid Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- FEMHKGDQDP QIAAHVISEA SSKTASVLQW APKGYYTLST NLVTLENGRQ LAVKRQGIYY IYAQVTFCSN RDAAGQAPFI ASLCLRSPSG SERILLRAAN THSSSKPCGQ QSIHLGGVFE LQPGASVFVN VTDPSQVSHG TGFTSFGLLK L Predicted Isoelectric Point: 6.62 Predicted Molecular Mass: 17.7kD Accurate Molecular Mass: 27kD as determined by SDS-PAGE reducing conditions Phenomenon Explanation: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Molekulargewicht:
- 17.7
- Physischer Zustand:
- Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
- Reinheit:
- ≥95%
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- -70[o]C
- Hersteller:
- United States Biological
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins