FGF-10, Recombinant, Rat, aa37-215 (KGF-2, Fibroblast Growth Factor)
Produktgrößen
25ug
£825,00
145700-25UG
Über dieses Produkt
- SKU:
- 145700
- Weitere Details:
- The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF10 belongs to the subgroup of FGFs that also includes FGF3, 7, and 22 (1). Mature rat FGF10 is an ~20kD protein that contains a serinerich region near its Nterminus (2). It shares 96% and 91% amino acid sequence identity with human and mouse FGF10, respectively. FGF10 is secreted by mesenchymal cells and associates with extracellular FGFBP (1, 3). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (4). FGF10 plays an important role in many tissues during embryogenesis. This includes limb bud initiation (5), palate development (6), branching morphogenesis and directional outgrowth of lung buds (7, 8), formation of the otic vesicle and chochlea (9), adipogenesis (10), folliculogenesis (11), cardiomyocyte differentiation (12), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (1316). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (6, 9, 1416). The expression and function of FGF10 are negatively regulated by Shh and BMP4 in the developing lung (7, 8). Overlapping expression patterns and activities with FGF3, 7, and 8 suggest at least a partial redundancy in FGF10 biology (6, 8, 15, 16). FGF10 signaling through FGF RIIIb also contributes to the progression of pancreatic cancer (17). Source: Recombinant protein corresponding to aa37-215 from rat FGF-10 expressed in E. coli. Molecular Weight: ~22kD Endotoxin: <0.01EU/1ug (LAL method). Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molekulargewicht:
- 22
- Physischer Zustand:
- Supplied as a lyophilized powder in MOPS, Na2SO4, EDTA, DTT. BSA free. Reconstitute in 250ul sterile PBS.
- Reinheit:
- ~95% (SDS-PAGE)
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins