Wnt10B (Wnt-10b, Wnt-12)
Produktgrößen
100ug
£748,00
145655-100UG
Über dieses Produkt
- SKU:
- 145655
- Anwendung:
- Immunohistochemistry
- translate.label.attr.clone:
- 793116
- Klonalität:
- Monoclonal
- Weitere Details:
- Wnt10b (also known as Wnt12) is a 4244kD member of the Wnt family of secreted, highly conserved, cysteinerich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (13). Human Wnt10b cDNA encodes a 389 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). Human Wnt10b shares 9799% aa identity with mouse, rat, equine, porcine, and canine Wnt10b. Wnt10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (57). Mouse Wnt10b deletion produces agedependent loss of bone mass due to defective production of osteoblasts, while transgenic overexpression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (57). Ectopic expression of Wnt10b in an obesity and diabetesprone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a missense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe earlyonset obesity (10, 11). Wnt10b is mainly produced by stem cells and preosteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt10b, are expressed in both normal and/or malignant colon tissues (14). Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8-25ug/ml with immersion fixed paraffin-embedded sections of human breast cancer tissue. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa29-389 from human Wnt-10b, CHO-derived.
- Isotyp:
- IgG2a
- Physischer Zustand:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Reinheit:
- Purified by Protein A affinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human Wnt10b. Species Crossreactivity: 100% recombinant mouse Wnt10b.
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody