PDCD1 (Programmed Cell Death 1, PD-1, CD279)
Produktgrößen
100ug
£856,00
145564-100UG
Über dieses Produkt
- SKU:
- 145564
- Anwendung:
- Flow Cytometry
- translate.label.attr.clone:
- 766104
- Klonalität:
- Monoclonal
- Weitere Details:
- Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Other members of this family include CD28, CTLA-4, and ICOS (2-4). PD-1 is most closely related to CTLA-4 and shares ~24% amino acid (aa) sequence identity. The mouse PD1 gene encodes a 288aa protein with a putative 20aa signal peptide, a 149aa extracellular region with one immunoglobulin-like V-type domain, a 21aa transmembrane domain, and a 98aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share ~69% aa sequence identity. Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2, have been identified as PD-1 ligands (5, 6). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Binding of PD-1 to PD-L1 or PD-L2 results in the inhibition of TCR-mediated proliferation and cytokine production as well as BCRmediated signaling. PD-1 likely has an inhibitory role in regulating immune responses (1-4). Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to mouse PDCD1, NS0-derived (UniProt # Q02242).
- Isotyp:
- IgG2a
- Physischer Zustand:
- Supplied as a lyophilized powder in PBS, 5% Trehalose. Reconstitute in 200ul sterile PBS.
- Reinheit:
- Purified by Protein A or G affinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes mouse PDCD1 in direct ELISAs, no crossreactivity with recombinant human PD1 is observed.
- Quelle:
- mouse
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody