Granzyme K (Fragmentin-3, Granzyme 3, GZMK, NK-Tryp-2, NK-Tryptase-2, PRSS, TRYP2, Tryptase II)
Produktgrößen
100ug
£825,00
052832-100UG
Über dieses Produkt
- SKU:
- 052832
- Anwendung:
- Flow Cytometry, Immunohistochemistry, Western Blot
- translate.label.attr.clone:
- 2471A (recomb)
- Klonalität:
- Monoclonal
- Weitere Details:
- Granzymes are released by cytoplasmic granules within NK and cytotoxic T cells. They are serine proteases that induce apoptosis in the target cell. Granzymes have also been found to help initiate the inflammatory response by activating macrophages and mast cells when in an extracellular state. Granzymes have also been found to protect the body against the formation of different kinds of lymphomas. Applications: Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 25ug/ml using human peripheral blood mononuclear cells (PBMCs). Western Blot: 0.1ug/ml with lysates of human NK cells, U2OS human osteosarcoma cell line, and RT-4 human bladder carcinoma cell line under reducing conditions. Immunohistochemistry (paraffin): 5-25ug/ml in immersion fixed paraffin-embedded sections of human tonsil. Requires HIER. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to human Granzyme K.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Reinheit:
- Purified by Protein A/G affinity chromatography from cell culture supernatant.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human Granzyme K.
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody