LDL Receptor (Low-Density Lipoprotein Receptor)
Produktgrößen
100ug
£761,00
052642-100UG
Über dieses Produkt
- SKU:
- 052642
- Anwendung:
- Flow Cytometry, Immunohistochemistry, Western Blot
- translate.label.attr.clone:
- 2H7.1
- Klonalität:
- Monoclonal
- Weitere Details:
- Low-density lipoprotein receptor (UniProt P01130; also known as LDL receptor) is encoded by the LDLR (also known as FH, FHC) gene (Gene ID 3949) in human. Low-density lipoprotein (LDL) receptor recognizes the apoprotein B100 embedded in the outer phospholipid layer of cholesterol-rich LDL particles and mediates their remval by endocytosis via clathrin-coated pits. LDL receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). LDLR gene mutations are known causes of familial hypercholesterolaemia (FH). LDL receptor is initially produced with a signal peptide sequence (a.a. 1-21), the removal of which yields the mature single transmembrane (a.a. 789-810) protein with a C-terminal cytoplasmic tail (a.a. 811-860) and a large N-terminal extracellular region (a.a. 22-788) that contains seven class A (a.a. 25-313) and six class B (a.a. 397-658) LDLR repeats, as well as three EGF-like domains (a.a. 314-353, 354-393, 663-712). LDL-A repeats mediate LDL binding, repeats 2-7 are required for ApoB binding, while only repeat 5 is required for ApoE binding. The class B (LDL-B) repeats region adopts a beta-propeller conformation and is believed to be responsible for the pH-dependent release of LDL in the endosome. The cytoplasmic tail contains a signal sequence important for localizing the receptors to clathrin-coated pits and for triggering receptor-mediated endocytosis upon LDL binding. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml detected LDL receptor in 10ug of human adrenal gland tissue lysate. Immunohistochemistry (paraffin): 1:250 detected LDL receptor immunoreactivity in human adrenal gland tissue. Requires HIER. Flow Cytometry: 1ug detected LDL receptor expression in 10e6 HepG2 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- GST-tagged recombinant protein of human LDL receptor extracellular fragment corresponding to class A domains 1-3.
- Isotyp:
- IgG2b,k
- Physischer Zustand:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Reinheit:
- Purified by Protein G affinity chromatography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes human LDL Receptor. Expected to react with spliced isoforms 1 and 5. Reactivity toward spliced isoforms 2, 3, 4, and 6 has not been determined.
- Quelle:
- human
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Monoclonal Antibody