IgG (HRP)
Produktgrößen
100ul
£371,00
048954-100UL
Über dieses Produkt
- SKU:
- 048954
- Anwendung:
- Western Blot
- Klonalität:
- Polyclonal
- Weitere Details:
- Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:3000, incubate membrane with diluted primary antibody in TBS, 5% nonfat dry milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Isotyp:
- IgG
- Physischer Zustand:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP).
- Reinheit:
- Purified by affintiy chromotography.
- Versandbedingungen:
- Blue Ice
- Spezifität:
- Recognizes mouse IgG.
- Quelle:
- mouse
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- United States Biological
- Typ:
- Antibodies:Polyclonal Antibody