Di-ubiquitin Explorer Kit
Produktgrößen
1 Kit
£692,00
J2000-1KIT
Über dieses Produkt
- SKU:
- J2000
- Buffer:
- 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
- Weitere Details:
- This kit is designed for two purposes: 1) identification of proteins that specially bind K48 polyUb chains in whole cell or tissue lysates; 2) validation of direct interactions between K48 polyUb chains and their binding proteins. The Non-cleavable K48 polyUb chain (2-4) are resistant of deubiquitination when incubating with whole cell or tissue lysates, thus allowing maximally capture proteins that specifically bind K48 polyUb chains. After binding, 6xHis-Non-cleavable K48 polyUb chains and the binding proteins can be enriched by Nickel XPure Agarose Resin (included). The bound proteins and the non-cleavable K48 polyUb chains can be eluted from Ni resin using a buffer containing 250 mM imidazole (included) or by incubating with thrombin (not included). Either of the elution method can significantly reduce the amounts of non-specific binding proteins when compared with elution using SDS sample buffer.
- Versandbedingungen:
- Dry Ice
- Quelle:
- E. coli recombinant
- Lagerbedingungen:
- - 80 0C
- Hersteller:
- UBP Bio
- Typ:
- Assay Kits: Assay Kits