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Revitablot[TM] Western Blot Stripping Buffer

Produktgrößen
500 ml
£164,00
MB-085-0500-500ML
Über dieses Produkt
SKU:
MB-085-0500
Zusätzliche Namen:
Stripping solution, Western Blot stripping buffer, western blot reprobing buffer, bound antibody removal buffer
Anwendung:
Western Blot
CE/IVD:
RUO
Konzentration:
1X
Weitere Details:
Revitablot™ Western Blot Stripping Buffer is a uniquely formulated, ready-to-use reagent specifically designed for western blotting. Revitablot™ Western Blot Stripping Buffer is faster and more efficient at stripping of primary and secondary antibodies, including HRP and fluorescent labeled. Blots can be stripped multiple times for the repeated use of membranes. This solution can be used at room temperature. Only incubation of 5-20 minutes needed to strip the membrane. Revitablot™ Western Blot Stripping Buffer is gentle and effective on both nitrocellulose and PVDF western blot membranes.
Physischer Zustand:
Liquid
Versandbedingungen:
Ambient
Spezifität:
Revitablot™ Western Blot Stripping Buffer contains solutions in a proprietary combination to enhance the removal of bound antibodies from western blot membranes for repeated use. The proprietary formulation of the solution ensures high stripping efficiency with low backgrounds.
Lagerbedingungen:
RT
Hersteller:
Rockland Inc
Typ:
Buffers & General Consumables: Others
Revitablot™ Western Blot Stripping Buffer
Western Blot comparison of Revitablot™ Western Blot Stripping Buffer. Lane 1: 70 ug HeLa whole cell lysates. Lane 2: 10 ug HeLa whole cell lysates. Blot A first probed with: Primary antibody: Rabbit Anti-STAT5 (p/n 600-401-A44) at a dilution of 1:1,000 overnight at 4°C. Secondary antibody: HRP Goat anti-rabbit (p/n 611-103-122) at 1:10,000 dilution using FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX-110). Membranes were then incubated with Revitablot and a competitor's product for 20 minutes at room temperature, followed by re-blocking with 5% BLOTTO for 2 hours at room temperature. Blot B (Blot A reprobed) with: Primary antibody: Rabbit anti-tubulin (p/n 600-401-880) at a dilution of 1:1,000 overnight at 4°C. Secondary antibody: HRP Goat anti-rabbit (p/n 611-103-122) at 1:10,000 dilution using FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX-110).
Western Blot comparison of Revitablot™ Western Blot Stripping Buffer using Fluorescent labeled Primary Antibodies. Lane 1: 7.5 ug HCT-116 cell lysates. Lane 2: 22.5 ug HCT-116 cell lysates. Lane 3: 15.0 ug HCT-116 cell lysates. Block: 5% BLOTTO for 1 hr at room temperature. Blot A first probed with: Primary antibody: Mouse anti-AKT pS473 (p/n 200-343-268) at a dilution of 1:1,000 overnight at 4°C. Imaging: 650nm. Membranes were then incubated with Revitablot™ 5 minutes at room temperature, followed by re-blocking with 5% BLOTTO for 2 hours at room temperature. Blot B (Blot A reprobed) with: Primary antibody: Rabbit anti-beta actin DyLight 488 Conjugated at a dilution of 1:1,000 for 4 hours at room temperature. Imaging: 488nm and 650nm.