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Antikörper

anti-Glial Filament Protein, guinea pig polyclonal antibody

Produktgrößen
100 ul
£364,00
GP52-100UL
Über dieses Produkt
SKU:
GP52
Zusätzliche Namen:
Glial fibrillary acidic protein, GFAP, GFAP
Anwendung:
Immunohistochemistry
CE/IVD:
RUO
Klonalität:
Polyclonal
Weitere Details:
Specific detection of GFAP (Glial Fibrillary Acidic Protein, Glial Filament Protein) (Mr 52000 polypeptide). Tumors specifically detected: Astrocytomas, gangliomas, meulloblastomas, certain teratomas
Formulierung:
Contains 0.09% sodium azide and 0.5% BSA
Host:
Guinea Pig
Immunogen:
Gliafilament protein purified from bovine spinal cord
Aufreinigung:
Stabilized antiserum
Reaktivitäten:
Mouse, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Please refer to datasheet
Typ:
Antibody: Polyclonal Antibody
  • Datenblatt des Herstellers:GP52
IHC analysis of mouse brain using anti-glial fibrillary acidic protein antibody (Cat. No. GP52). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-GFAP (Cat. No. GP52) was diluted in PBS (1:2000) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratries) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The picture was acquired using microscopy (courtesy of J.Hess
University Hospital Heidelberg).
IHC analysis of mouse spinal cord using anti-glial fibrillary acidic protein antibody (Cat. No. GP52). IHC was performed on formalin fixed frozen sections (12 um). Heat induced antigen retrieval was performed in citrate buffer pH 6.0 for 1 h at 65°C. After preparation the tissue was blocked with 5% donkey serum in fish skin buffer for 1 h at RT. The primary antibody anti-GFAP (Cat. No. GP52) was diluted in fish skin buffer (1:500) and incubated at RT over-night. The secondary antibody donkey anti-guinea pig Alexa488 was also diluted in fish skin buffer (1:1