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Antikörper

anti-Perilipin 2 (N-terminus), guinea pig polyclonal antibody

Produktgrößen
100 ul
£384,00
GP46-100UL
Über dieses Produkt
SKU:
GP46
Zusätzliche Namen:
Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP
Anwendung:
Immunocytochemistry, Immunohistochemistry, Western Blot
CE/IVD:
RUO
Klonalität:
Polyclonal
Weitere Details:
Adipophilin / ADRP / PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (Heid et al. 1998); inducible by etomoxir. Enhanced expression of Adipophilin / ADRP / PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry of e.g. HCV via lipid droplets (Hope et al.2002). Polypeptide reacting: Adipophilin / ADRP / PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72. Tissue immunolocalization: Adipophilin / PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages. Reactivity on cultured cell lines: PLC. Hope, R. G., Murphy, D. J. & McLauchlan, J. The Domains Required to Direct Core Proteins of Hepatitis C Virus and GB Virus-B to Lipid Droplets Share Common Features with Plant Oleosin Proteins. J. Biol. Chem. 277, 4261-4270 (2002). Heid, H. W., Moll, R., Schwetlick, I., Rackwitz, H. R. & Keenan, T. W. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res. 294, 309-21 (1998).
Formulierung:
Contains 0.09% sodium azide and 0.5% BSA
Host:
Guinea Pig
Immunogen:
Synthetic peptide (N-terminal aa 1-16 of human adipophilin / PLIN2)
Aufreinigung:
Stabilized antiserum
Reaktivitäten:
Human, Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Please refer to datasheet
Typ:
Antibody: Polyclonal Antibody
  • Datenblatt des Herstellers:GP46
Western blot analysis of undifferentiated 3T3-L1 and differentiated 3T3-L1 ALC (= adipocyte like cells) cell lysate with anti-Perilipin 2 antibody (Cat. No. GP46). The cells were differentiated using medium containing 0.5 mM IBMX
1 uM dexamethanosone and 10 ug/ml insulin and lysed with RIPA buffer. Western blot analysis was performed on 70 ug of cell lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibodies anti-Perilipin 2 (Cat. No. GP46) and anti-GAPDH were diluted in blocking buffer (1:500 Anti-Perilipin
1:1
500 anti-GAPDH) and incubated at 4°C over-night. The secondary antibodies donkey anti-guinea pig A647 and goat anti-mouse A488 were also diluted in blocking buffer (both 1:300) and incubated for 1 h at RT. The bands were visualized by fluorescent detection.
Western blot analysis of HepG2 lysate with anti-Perilipin 2 antibody (Cat. No. GP46). Western blot analysis was performed on 30 ug of HepG2 lysate and 39 ug HepG2 + OA cells. The cells were previously treated with 400 ug oleic acid (OA) if indicated. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibodies anti-Perilipin 2 (Cat. No. GP46) and anti-GAPDH were diluted in blocking buffer (1:500 Anti-Perilipin