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Antikörper

anti-Cyclin D1, DCS-6, mouse monoclonal antibody

Produktgrößen
50 ug
£224,00
61053-50UG
Über dieses Produkt
SKU:
61053
Anwendung:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
DCS-6
Klonalität:
Monoclonal
Konzentration:
50 ug/ml
Weitere Details:
DCS-6 reacts specifically with cyclin D1 protein present predominantly in G1 phase of cell cycle; it does not cross-react with D2 or D3 cyclins (the closest relatives within the cyclin family); DCS-6 is especially useful in tumor diagnosis: it can be used as fast method to identify cases with 11q13 amplifications. Cyclin D1 gene is identical to the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma. Polypeptide reacting: Mr 36 kDa polypeptide (cyclin D1 polypeptide of human cells). Reactivity on cultured cell lines: MCF-7, MDA-MB-134 and MDA-MB-231 (breast cancer cell lines), UMSC22 (squamous cell carcinoma cell line), U-2-OS (osteosarcoma cell line), RT-112, A431, HT-29, 3T3-L1, Ins-1. The expression of Cyclin D1 is tissue specific and associated with tumor onset and progression.
Formulierung:
Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Host:
Mouse
Immunogen:
Human recombinant full-length cyclin D1 protein
Isotyp:
IgG2a
Physischer Zustand:
Lyophilized
Aufreinigung:
Affinity Purified
Reaktivitäten:
Canine, Human, Mouse, Non-human Primate, Rat
Versandbedingungen:
Blue Ice
Lagerbedingungen:
2-8[o]C reconstituted. Aliquot. Avoid freeze/thaw cycles.
Typ:
Antibody: Monoclonal Antibody
  • Datenblatt des Herstellers:61053
Western blot analysis of mouse 3T3-L1 and rat Ins-1 cell lysate with anti-Cyclin D1 antibody. Western blot analysis was performed on 20 ug of either 3T3 or Ins-1 lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Cyclin D1 mouse monoclonal
DCS-6 (Cat. No. 690053) was diluted in blocking buffer (antibody concentration 2 ug/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal
HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 ug/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
IHC analysis of head and neck squamous cell carcinoma using anti-Cyclin D1. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Cyclin D1 mouse monoclonal