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Antikörper

Anti-Semaphorin-6A (cytoplasmic region) Antibody

Produktgrößen
100 ul
£433,00
SP5371-100UL
Über dieses Produkt
SKU:
SP5371
Anwendung:
ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Klonalität:
Polyclonal
Weitere Details:
Our Semaphorin-6A (cytoplasmic region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Semaphorin-6A (cytoplasmic region) and is antigen affinity purified. It is great for use in WB, ICC, IP., ECM
Format:
Antigen Affinity Purified
Formulierung:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gendetails:
SEMA6A
Host:
Rabbit
Immunogen:
Semaphorin 6A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the cytoplasmic region of human Sema-6A. The sequence used has high homology to similar regions in rat and mouse Sema-6A, and has low homology to other semaphorin family members.
Isotyp:
IgG
Molekulargewicht:
120
Physischer Zustand:
Liquid
Aufreinigung:
Affinity purified rabbit serum
Reaktivitäten:
Amphibian, Avian, Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Spezifität:
The antibody detects a 120kDa* protein corresponding to the apparent molecular mass of Sema-6A on SDS-PAGE immunoblots of Sema-6A transfected COS7 cells, human colorectal cancer cells, and rat PC12 cells. In addition, shRNA knockdown of Sema-6A expression significantly reduces detection of Sema-6A using anti-Sema-6A (a.a. 772-787) antibody.
Lagerbedingungen:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Typ:
Antibody: Polyclonal Antibody
Immunocytochemical labeling of Sema-6A in COS7 cells that were mock transfected (top images) or Sema-6A transfected (bottom images). The cells were labeled with anti-Sema-6A (a.a. 772-787) (Left top and bottom image) or anti-Sema-6A (C-terminus) (Right top and bottom image). The antibodies were detected using anti-rabbit fluorescent secondary antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, University of Torino, Italy).
Western blot of Sema-6A in human colorectal cancer (CRC) cells treated with control (shC) or Sema-6A (shSema6A) shRNAs. Sema-6A was immunoprecipitated from each of the CRC lysates using Sema-6A (a.a. 772-787) or Sema-6A (C-terminus) antibody, then the blotted immunoprecipitations were probed with Sema-6A antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, Univ. of Torino, Italy).