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Antikörper

Anti-Robo1 (C-terminal region) Antibody

Produktgrößen
100 ul
£372,00
RP2791-100UL
Über dieses Produkt
SKU:
RP2791
Anwendung:
ELISA, Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Klonalität:
Polyclonal
Weitere Details:
Our Robo1 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Robo1 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC., ECM
Format:
Antigen Affinity Purified
Formulierung:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gendetails:
ROBO1
Host:
Rabbit
Immunogen:
Robo1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human Robo1. This sequence has significant homology to the same region in rat and mouse Robo1, and has low homology to other Robo family members.
Isotyp:
IgG
Molekulargewicht:
180
Physischer Zustand:
Liquid
Aufreinigung:
Affinity purified rabbit serum
Reaktivitäten:
Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Spezifität:
The antibody detects a 180 kDa* band corresponding to Robo1 in Western blots of human HepG2 and HUVEC cells, as well as mouse C2C12 cells.
Lagerbedingungen:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Typ:
Antibody: Polyclonal Antibody
Western blot analysis of HepG2 (lanes 1 & 4), C2C12 (lanes 2 & 5), and HUVEC (lanes 3 & 6). The blot was probed with anti-Robo1 (C-terminal region) in absence (lanes 1-3) or presence of Robo1 (C-terminal region) blocking peptide (RX2795; lanes 4-6).
Immunocytochemical labeling of Robo1 in mouse C2C12. The cells were labeled with rabbit polyclonal Robo1 (C-terminal region) in the absence or presence of blocking peptide (RX2795). The antibody was then detected using appropriate secondary antibodies conjugated to Cy3.