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Antikörper

Anti-p38A Alpha MAP Kinase (Tyr-323), Phosphospecific Antibody

Produktgrößen
100 ul
£433,00
PP3411-100UL
Über dieses Produkt
SKU:
PP3411
Zusätzliche Namen:
MAPK, p38, p38alpha, p38MAPK
Anwendung:
ELISA, Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Klonalität:
Polyclonal
Konjugat:
Tyr-323 - Phosphorylated
Weitere Details:
Our p38A Alpha MAP Kinase (Tyr-323) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat p38A Alpha MAP Kinase (Tyr-323) and is antigen affinity purified. It is great for use in WB, ICC., ECM
Format:
Antigen Affinity Purified
Formulierung:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gendetails:
Mapk14
Host:
Rabbit
Immunogen:
Phospho-p38A Alpha MAP Kinase (Tyr-323) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 323 in mouse p38A Alpha. This peptide sequence is highly conserved in human and rat p38A Alpha, and has high homology to the conserved site in p38B Beta.
Isotyp:
IgG
Molekulargewicht:
38
Physischer Zustand:
Liquid
Aufreinigung:
Affinity purified rabbit serum
Reaktivitäten:
Avian, Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Spezifität:
This antibody was cross-adsorbed to a phospho-tyrosine peptide before affinity purification using phospho-p38A Alpha (Tyr-323) peptide (without carrier). The antibody detects a 38 kDa* band corresponding to p38A Alpha on SDS-PAGE immunoblots of human Jurkat, K562, and mouse macrophage (J774A.1) cells treated with pervanadate. In addition, the antibody detects recombinant p38 MAP Kinase when treate
Lagerbedingungen:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Typ:
Antibody: Polyclonal Antibody
Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38A Alpha MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38A Alpha (a.a. 319-328) (lanes 1 & 2) or anti-p38A Alpha (Tyr-323) (lanes 3 & 4).