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Antikörper

Anti-GluR1-Subunit (Ser831) Antibody

Produktgrößen
25 ul
£289,00
p1160-831-25UL
150 ul
£552,00
p1160-831-150UL
Über dieses Produkt
SKU:
p1160-831
Zusätzliche Namen:
GLUR 1 antibody, GLUR A antibody, AMPA 1 antibody, AMPA selective glutamate receptor 1 antibody, AMPA-selective glutamate receptor 1 antibody, GluA1 antibody, GLUH1 antibody, GluR K1 antibody, GluR-1 antibody, GluR-A antibody, GluR-K1 antibody, GLUR1 antibody, GLURA antibody, GluRK1 antibody, Glutamate receptor 1 antibody, Glutamate receptor ionotropic AMPA 1 antibody, Glutamate receptor ionotropic antibody, Glutamate receptor, ionotropic, AMPA 1 antibody, Gria1 antibody, GRIA1_HUMAN antibody, HBGR1 antibody, MGC133252 antibody, OTTHUMP00000160643 antibody, OTTHUMP00000165781 antibody, THUMP00000224241 antibody, OTTHUMP00000224242 antibody, OTTHUMP00000224243 antibody
Anwendung:
ELISA, Western Blot
Buffer:
10 mM HEPES (
CE/IVD:
RUO
Klonalität:
Polyclonal
Konjugat:
Ser831 - Phosphorylated
Weitere Details:
Our Anti-GluR1-Subunit (Ser831) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat GluR1-Subunit (Ser831) and is antigen affinity purified from pooled serum. It is great for use in WB., Phosphosolutions
Format:
Antigen Affinity Purified from Pooled Serum
Formulierung:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
Gendetails:
GRIA1
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser831 of rat GluR1 conjugated to keyhole limpet hemocyanin (KLH).
Isotyp:
IgG
Molekulargewicht:
100
Physischer Zustand:
Liquid
Aufreinigung:
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Reaktivitäten:
Avian, Canine, Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Spezifität:
Specific for endogenous levels of the ~100 kDa GluR1 protein phosphorylated at Ser831. Immunolabeling is completely eliminated by treatment with L Lambda-phosphatase.
Lagerbedingungen:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Typ:
Antibody: Polyclonal Antibody
Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring AS283-mediated inhibition of CaMKII phosphorylation of GluA1 S831 (pS831) (cat. p1160-831, 1:2000) and autophosphorylation of CaMKII T286 (pT286) (cat. p1005-286, 1:2500) with varying concentrations of AS283 (0.41-10 uM). For a negative control (Neg.), ATP was omitted. Image from publication CC-BY-4.0. PMID: 37648853
Representative Immunoblots and quantification of in vitro kinase reactions at 30 °C with increasing ATP concentration measuring purified CaMKIIA Alpha phosphorylation of GST-GluA1 S831 (cat. p1160-831, 1:2000). Image from publication CC-BY-4.0. PMID: 37648853
Immunoblots of in vitro kinase reactions at 30 °C including GFP-CaMKII WT, K42M and F89G with GST-GluA1-c tail showing specific labelling of GluR1 S831 (cat. p1160-831, 1:2000). Reactions were performed either with vehicle control or with 10 uM NM-PP1. Image from publication CC-BY-4.0. PMID: 37648853
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (L Lambda-Ptase, 1200 units for 30 min).