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Antikörper

Anti-CaM Kinase II (Thr286) Antibody

Produktgrößen
25 ul
£289,00
p1005-286-25UL
100 ul
£552,00
p1005-286-100UL
Über dieses Produkt
SKU:
p1005-286
Zusätzliche Namen:
Calcium/calmodulin dependent protein kinase II alpha antibody, Calcium/calmodulin dependent protein kinase II beta antibody, Calcium/calmodulin dependent protein kinase II delta antibody, Calcium/calmodulin dependent protein kinase II gamma antibody, Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody, CaM kinase II alpha antibody, CaM kinase II antibody, CaM kinase II beta antibody, CaM kinase II delta antibody, CaM kinase II gamma antibody, CaM kinase II subunit alpha antibody, CaMK-II subunit alpha antibody, CAMK2 antibody, Camk2a antibody, CAMK2B antibody, CAMK2D antibody, CAMK2G antibody, CAMKA antibody, KCC2A_HUMAN antibody
Anwendung:
ELISA, Immunohistochemistry, Western Blot
Buffer:
10 mM HEPES (
CE/IVD:
RUO
Klonalität:
Polyclonal
Konjugat:
Thr286 - Phosphorylated
Weitere Details:
Our Anti-CaM Kinase II (Thr286) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, rabbit, and rat CaM Kinase II (Thr286) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC., Phosphosolutions
Format:
Antigen Affinity Purified from Pooled Serum
Formulierung:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
Gendetails:
CAMK2A/B
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH).
Isotyp:
IgG
Molekulargewicht:
50/60
Physischer Zustand:
Liquid
Aufreinigung:
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Reaktivitäten:
Fish, Human, Mouse, Rabbit, Rat
Versandbedingungen:
Blue Ice
Spezifität:
Specific for endogenous levels of the ~50 kDa A Alpha-CaM Kinase II and the ~60 kDa B Beta-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is completely eliminated by treatment with L Lambda-phosphatase.
Lagerbedingungen:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Typ:
Antibody: Polyclonal Antibody
Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa A Alpha- and the ~60 kDa B Beta-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (L Lambda-Ptase, 1200 units for 30 minutes).
Astrocytes (1 x 106 cells/ml) were cultured for 24 h without treatment or with METH (10 uM), clade B gp120 (50 ng/ ml), or clade C gp120 (50 ng/ ml) alone or METH in combination with clade B or C gp120. After incubation, the cells were fixed, permeabilized, and stained for (A) DRD-2, (B) phospho-CaMK II (cat. p1005-286) (C) phospho-CaMK IV (green) or GFPA (red) and DAPI (blue). The cells were imaged using a confocal microscope at 630x and analyzed using immunocytochemistry. Image from publication CC-BY-4.0. PMID: 26057350
Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring CaMKIIA Alpha WT and F89G phosphorylation of T286 (cat. p1005-286, 1:2500). Image from publication CC-BY-4.0. PMID: 37648853
Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring AS283-mediated inhibition of CaMKII phosphorylation of GluA1 S831 (pS831) (cat. p1160-831, 1:2000) and autophosphorylation of CaMKII T286 (pT286) (cat. p1005-286, 1:2500) with varying concentrations of AS283 (0.41-10 uM). For a negative control (Neg.), ATP was omitted. Image from publication CC-BY-4.0. PMID: 37648853
Depicts increased CaMKII autophosphorylation at Threonine 287 (cat. p1005-286, 1:1000) (normalized to CaMKIID Delta expression) following ATX-II exposure in WT and KI cells. Image from publication CC-BY-4.0. PMID: 39077112