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Antikörper

Anti-ERK1/2 (MAPK) (Thr-202/Tyr-204) [conserved], Phosphospecific Antibody

Produktgrößen
100 ul
£467,00
EM2061-100UL
Über dieses Produkt
SKU:
EM2061
Zusätzliche Namen:
ERK, p42, p44, MAPK
Anwendung:
ELISA, Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
M206
Klonalität:
Monoclonal
Konjugat:
- GOmAb/Phosphorylated
Weitere Details:
Our ERK1/2 (MAPK) (Thr-202/Tyr-204) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat ERK1/2 (Thr-202/Tyr-204) and is protein a purified. It is great for use in ELISA, WB, ICC., ECM
Format:
Protein A Purified
Formulierung:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gendetails:
MAPK1
Host:
Mouse
Immunogen:
Clone M206 was generated from a dual phosphorylated ERK1 (Thr-202/Tyr-204) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-202 and Tyr-204 in human ERK1. This sequence is conserved in rat and mouse ERK1, and is highly conserved in ERK2.
Isotyp:
IgG1
Molekulargewicht:
42/44
Physischer Zustand:
Liquid
Aufreinigung:
Protein A purified culture supernatant.
Reaktivitäten:
Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Spezifität:
The antibody detects 42 and 44 kDa* proteins corresponding to ERK1 and ERK2 on SDS-PAGE immunoblots of human A431 epithelial cells stimulated with EGF or calyculin A. It does not detect bands in control cells.
Lagerbedingungen:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Typ:
Antibody: Monoclonal Antibody
Immunocytochemical labeling of phosphorylated ERK1 in paraformaldehyde-fixed and NP-40-permeabilized rat A7r5 cells treated with calyculin A. The fixed cells were labeled with mouse monoclonal antibodies to anti-ERK1 (EM2331) and anti-ERK1/2 (Thr-202/Tyr-204) (EM2061). The antibodies were detected using Goat anti-Mouse secondary antibodies conjugated to DyLight® 488 (left) and DyLight® 594 (right).
Western blot analysis of human A431 epithelial cells untreated (lanes 1 & 4) or treated with 100 nM calyculin A for 30 min. (lanes 2 & 5) or 100 ng/ml EGF for 60 min. (lanes 3 & 6). The blots were probed with anti-ERK1 (C-terminal region) (lanes 1, 2, & 3) or anti-ERK1/2 (Thr-202/Tyr-204) (lanes 4, 5, & 6).