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Antikörper

Anti-Atrogin-1 Antibody

Produktgrößen
100 ul
£433,00
AP2041-100UL
Über dieses Produkt
SKU:
AP2041
Zusätzliche Namen:
MAFbx, FBX32, Atrogin
Anwendung:
ELISA, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Klonalität:
Polyclonal
Weitere Details:
Our Atrogin-1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Atrogin-1 and is antigen affinity purified. It is great for use in WB, IHC., ECM
Format:
Antigen Affinity Purified
Formulierung:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gendetails:
Fbxo32
Host:
Rabbit
Immunogen:
A synthetic peptide (coupled to carrier protein) corresponding to amino acids from the N-terminal region of mouse Atrogin. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32).
Isotyp:
IgG
Molekulargewicht:
41
Physischer Zustand:
Liquid
Aufreinigung:
Affinity purified rabbit serum
Reaktivitäten:
Human, Mouse, Rat
Versandbedingungen:
Blue Ice
Spezifität:
This antibody detects a 41 kDa* protein corresponding to the apparent molecular mass of Atrogin on SDS-PAGE immunoblots of mouse muscle tissue lysates. Similar results were seen in C2C12 cell lysate and the band observed in western blot is specifically blocked by Atrogin-1 peptide (AX2045).
Lagerbedingungen:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Typ:
Antibody: Polyclonal Antibody
Formalin fixed, citric acid treated paraffin sections of E16 mouse skeletal muscle. Sections were probed with anti-Atrogin-1 (AP2041) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
Western blot analysis of MuRF1 (cat. MP3401), Atrogin-1 (cat. AP2041), FoxO3a, p-AKT (Ser473), and total AKT in the soleus muscle of control, HU, and HU+TUDCA mice. Image from publication CC-BY-4.0. PMID: 37525907
Western blot image of mouse gastrocnemius (lanes 1 & 3) and mouse diaphragm tissue lysate (lanes 2 & 4). The blot was probed with anti-Atrogin-1 (AP2041; lanes 1-4) in the presence (lanes 3 & 4) or absence (lanes 1 & 2) of Atrogin-1 peptide (AX2045).
Western blotting analysis of the expression of Atrogin-1 (cat. AP2041, 1:1000) and MuRF1 (cat. MP3401, 1:1000) proteins in the soleus muscles after HS. Image from publication CC-BY-4.0. PMID: 37344807
Western blot showing MuRF-1( cat MP3401, 1:500) and atrogin-1 (cat. AP2041), 1:500) levels in C2C12 myoblasts differentiated for 4-5 days were incubated with 200 uM UDCA for 72 h. GAPDH was used as a loading control. Image from publication CC-BY-4.0. PMID: 37237400