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Assay-Kits & ELISAs

Fluorescent Cell Proliferation Assay

Produktgrößen
1000 tests
£412,00
INDBLU100-4P-1000TESTS
Über dieses Produkt
SKU:
INDBLU100-4P
Zusätzliche Namen:
Metabolic Assay
Anwendung:
ELISA
CE/IVD:
RUO
Weitere Details:
An oxidation/reduction-based reagent quantitatively measures the reducing power of living cells. The cell-permeable reagent is blue in color, but non-fluorescent. When reduced by viable cells, it fluoresces red. Read in a fluorescent plate reader., ICT
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C
Typ:
Assay Kits: Detection
Figure 1. Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat cells (10,000 cells/well) in 96-well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Dye reagent (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530 nm and Em: 590 nm.
Figure 2. PBMC proliferation in response to mitogen Concanavalin A (Con-A) stimulation measured using Fluorescent Cell Proliferation Assay. PBMC at 15,000 cells were cultured for 3 days in the presence of titrated amounts of Con A and tested for proliferation using the Fluorescent Cell Proliferation Assay. Dye reagent (1/10 volume) was added to the cultured cells and incubated at 37°C for 2h. Fluorescence was detected at Ex: 530 nm and Em: 590 nm.