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Assay-Kits & ELISAs

Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay

Produktgrößen
6 tests
£274,00
982-6TESTS
Über dieses Produkt
SKU:
982
Anwendung:
Flow Cytometry
CE/IVD:
RUO
Weitere Details:
A non-cytotoxic, cell-permeant fluorescent inhibitor of caspases optimized for use in whole live animals. The probe contains the preferred binding sequence for most caspases (Val-Ala-Asp or VAD), coupled to a SR dye and a FMK reactive entity., ICT
Immunogen:
Poly Caspases
Physischer Zustand:
Tissue
Aufreinigung:
IN VIVO APOPTOSIS DETECTION, SR-FLIVO was used to assess the effectiveness of arsenic trioxide (ATO) treatment on SCK mammary tumors in vivo. Using a window chamber to view tumors directly, SCK mammar
Versandbedingungen:
Blue Ice
Lagerbedingungen:
2-8[o]C
Typ:
Assay Kits: Cell Viability
Figure 1. Excitation and emission spectra. Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay inhibitor probe was reconstituted in DMSO, diluted in an aqueous buffer, and then analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 630 nm. The emission spectrum (orange) was generated using an excitation of 540 nm. The probe optimally excites at 550-580 nm and has a peak emission at 590-600 nm.
Figure 2. Mice were given either a 0Gy or 15Gy whole lung dose of gamma irradiation. SR in vivo probe (Cat. 982/983) was injected and allowed to circulate for 18 hours prior to sacrifice. Paraffin sections were prepared, nuclei stained with DAPI (blue), and lungs imaged with a fluorescence microscope. Caspase activity (red) was induced in irradiated mice compared to control animals. Data courtesy of E. Hernady (University of Rochester Medical Center, Rochester, NY).
Figure 3. Using a window chamber to view tumors directly, SCK mammary tumor cells were grown under the skin of A/J mice for 7 days. Mice were treated with ATO or a placebo. Mice were injected IV with SR in vivo probe and images captured 30 minutes later. Bright spots indicate high levels of caspase activity within the tumor. ATO treatment doubled the level of caspase activity. Data courtesy of Dr. R. Griffin, University of Minnesota.
Figure 4. SR in vivo probe was used for non-invasive imaging of apoptosis in live animals. COLO205 cells were injected S/C into female nude mice (A). After 27 days, mice were treated with a control (B) or TRAIL at 25 mg/Kg (C and D), then injected with SR in vivo probe to image caspase-positive tumor cells. TRAIL induced apoptosis within 1 hour post-treatment (C), and the level of apoptosis significantly increased by 4 hours (D). Data courtesy of Dr. P. Lassota, Caliper Life Sciences / Xenogen.