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Assay-Kits & ELISAs

Green Fluorescent Cathepsin B Assay

Produktgrößen
100 tests
£641,00
9152-100TESTS
Über dieses Produkt
SKU:
9152
Anwendung:
Flow Cytometry
CE/IVD:
RUO
Weitere Details:
The quenched substrate Rhodamine 110-(RR)2, fluoresces green upon cleavage by active cathepsins, allowing the monitoring of intracellular cathepsin activity in vitro using live whole cells. Analyze using flow cytometry or fluorescence microscopy., ICT
Immunogen:
Cathepsin B
Physischer Zustand:
Cell Culture
Aufreinigung:
Cathepsin activity in THP-1 monocytes. Intracellular cathepsin activity was detected in THP-1 monocytes that were either untreated to show baseline cathepsin activity (green histogram) or were treated
Versandbedingungen:
Blue Ice
Lagerbedingungen:
2-8[o]C, -20[o]C
Typ:
Assay Kits: Detection
73-051-n3-26-ihc-1
Figure 3. THP-1 monocytes were either untreated to show baseline cathepsin activity (green) or were treated with the cathepsin inhibitor CA-074 Me (blue) to reduce cathepsin activity. Cells were stained with R110-(RR)2 and analyzed by flow cytometry (488 nm excitation and 530/30 emission filter). Treatment with CA-074 Me decreased the fluorescence signal detected compared to the untreated cell population. Data courtesy of Dr. Kristi Strandberg (ICT 231:47-50).
Figure 4. Jurkat cells were treated with the cathepsin inhibitor, E-64d (lower), or were untreated (upper). Cells were then stained with R110-(RR)2, followed by Hoechst 33342, and then mounted onto coverslips and imaged. Intracellular localization of the hydrolyzed (fluorescent) R110 product was detected using EGFP (Ex 470/30, Em 530/50) and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. The overlay includes a brightfield image. Data courtesy of Dr. Kristi Strandberg (ICT 231:65-68).
Figure 5. THP-1 cells were treated with PMA (5 ng/mL for 48 hours) to become macrophage-like, and then exposed to the cathepsin inhibitor, CA-074 Me (lower), or were untreated (upper). Cells were then stained with R110-(RR)2 and Hoechst 33342. Cells were imaged using EGFP (Ex 470/30, Em 530/50) and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Data courtesy of Dr. Kristi Strandberg (ICT 231:69).