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Assay-Kits & ELISAs

Intracellular GSH Assay Kit

Produktgrößen
100 tests
£310,00
9137-100TESTS
Über dieses Produkt
SKU:
9137
Anwendung:
Flow Cytometry
CE/IVD:
RUO
Weitere Details:
This whole cell glutathione assay employs a proprietary thiol-sensitive dye, to monitor relative changes in the intracellular concentration of reduced glutathione (GSH). Analyze the fluorescent signal by flow cytometry., ICT
Immunogen:
Glutathione (GSH)
Physischer Zustand:
Cell Culture
Aufreinigung:
Measurement of Intracellular GSH Levels in Non-Induced and Induced Jurkat cell samples. Jurkat cells were treated with a negative control agent or staurosporine to induce oxidative stress. Samples wer
Versandbedingungen:
Blue Ice
Lagerbedingungen:
-20[o]C
Typ:
Assay Kits: Detection
Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (middle), then stained using Intracellular GSH Assay (cat. 9137). Cells were read on a flow cytometer using an FL1 99% (2 log) attenuation filter. The median fluorescence intensity of negative control cells was 425,971 in FL1-A (left: Negative), and 289,169 in the induced cells (middle: Positive), a decrease of >30%. Overlay: green: Negative, red: Positive. Data courtesy of Ms. T. Hanson, ICT, 216:52, 051612.