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Antikörper

ACO1 Rabbit polyclonal Antibody

Produktgrößen
100μl
£370,00
R1512-16-100UL
Über dieses Produkt
SKU:
R1512-16
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
Klonalität:
Polyclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Human ACO1 aa 1-273 / 889.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 98 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Polyclonal Antibody
Western blot analysis of ACO1 on different lysates with Rabbit anti-ACO1 antibody (R1512-16) at 1/2;000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-16) at 1/2;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
ICC staining ACO1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde; permeabilised with 0.25% Triton X100/PBS.
ICC staining ACO1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde; permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ACO1 antibody. Counter stained with hematoxylin.